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ERX1904923: Illumina MiSeq paired end sequencing
1 ILLUMINA (Illumina MiSeq) run: 349,257 spots, 174.6M bases, 79.1Mb downloads

Submitted by: European Molecular Biology Laboratory, Heidelberg, Germany (European Molecular Biology Laboratory, Heidelberg,)
Study: High-throughput screening of neutralizing epitopes on HIV particles using single-virus droplet microfluidics
show Abstracthide Abstract
Analyzing surface epitopes of single HIV particles holds great potential for the development of vaccine candidates. However, existing technologies do not allow corresponding screens at high-throughput. We present here a single-virus droplet-based microfluidics platform enabling sorting of HIV-1 particles at a throughput of >200 per second based on the expression of epitopes recognized by broadly neutralizing antibodies. We show that virus particles displaying these epitopes can be identified, sorted and analyzed by next generation sequencing (NGS): an approximately 1.5 × 10^7 fold enrichment of viral particles displaying neutralizing epitopes could be obtained in a single sort, thus opening the way for screening diverse virus libraries with optimal antigenic features for HIV vaccine candidates.
Sample: sample1to100mix
SAMEA93288418 • ERS1562499 • All experiments • All runs
Library:
Name: Library_8
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Construction protocol: Viral RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen). Reverse transcription was performed using SuperScript III First-Strand Synthesis System for RT-PCR (Thermo Fisher Scientific). cDNA was used to amplify fragments containing env mutation using Q5 High-Fidelity DNA Polymerase (New England Biolabs).
Runs: 1 run, 349,257 spots, 174.6M bases, 79.1Mb
Run# of Spots# of BasesSizePublished
ERR1842946349,257174.6M79.1Mb2017-04-16

ID:
3948461

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